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( A ) CLSM micrographs of fluorescent polystyrene spheres taken up by <t>RBCs.</t> Autofluorescence of the cells is shown in red, and particles are green (bar = 2 μm). The xy and xz projections allow clear differentiation between internalized (arrows) and extracellular (arrowheads) particles; open arrowheads mark the position of the projections. ( B, C <t>)</t> <t>TEM</t> micrographs showing uptake of 0.025 μm gold particles by RBCs; the particles are not membrane bound (arrows). ( B ) Bar = 1 μm. ( C ) Higher magnification of portion of ( B ); bar = 0.2 μm.
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( A ) CLSM micrographs of fluorescent polystyrene spheres taken up by RBCs. Autofluorescence of the cells is shown in red, and particles are green (bar = 2 μm). The xy and xz projections allow clear differentiation between internalized (arrows) and extracellular (arrowheads) particles; open arrowheads mark the position of the projections. ( B, C ) TEM micrographs showing uptake of 0.025 μm gold particles by RBCs; the particles are not membrane bound (arrows). ( B ) Bar = 1 μm. ( C ) Higher magnification of portion of ( B ); bar = 0.2 μm.

Journal: Environmental Health Perspectives

Article Title: Ultrafine Particles Cross Cellular Membranes by Nonphagocytic Mechanisms in Lungs and in Cultured Cells

doi: 10.1289/ehp.8006

Figure Lengend Snippet: ( A ) CLSM micrographs of fluorescent polystyrene spheres taken up by RBCs. Autofluorescence of the cells is shown in red, and particles are green (bar = 2 μm). The xy and xz projections allow clear differentiation between internalized (arrows) and extracellular (arrowheads) particles; open arrowheads mark the position of the projections. ( B, C ) TEM micrographs showing uptake of 0.025 μm gold particles by RBCs; the particles are not membrane bound (arrows). ( B ) Bar = 1 μm. ( C ) Higher magnification of portion of ( B ); bar = 0.2 μm.

Article Snippet: We examined ultrathin sections of RBCs incubated with gold particles in a Philips 300 TEM at 60 kV (Philips, Zurich, Switzerland).

Techniques: Membrane